Journal: Marine Drugs

Article Title: Assessment of Heparanase-Mediated Angiogenesis Using Microvascular Endothelial Cells: Identification of λ-Carrageenan Derivative as a Potent Anti Angiogenic Agent

doi: 10.3390/md15050134

Figure Lengend Snippet: Effect of the addition of MCF-7 heparanase-rich supernatant on HSkMEC pseudo-vessels formation. Cells were plated in Matrigel TM in the presence (or absence) of 10 µL of heparanase-rich supernatant from MCF-7 cultured in FBS-free medium under hypoxia for 48 h. Capillary network formation was visualized using a Zeiss Axiovert 200 M fluorescent inverted microscope and monitored for 21 h. Angiogenesis was observed after 9.5 h under hypoxia in FBS-free medium: in the absence ( a ); or in the presence ( b ) of heparanase-rich MCF-7 supernatant. ( c ) Angiogenesis formation was followed over time with pictures taken every 2 h and by studying the number of pseudovessels, junctions, meshes and the size of the meshes (indicated in pixels). Results are presented as the mean ± SD for triplicates.

Article Snippet: Cell rearrangement and pseudo-vessel formation were visualized using a Zeiss Axiovert 200M fluorescence inverted microscope (Zeiss, Le Pecq, France) equipped with an Axiocam high-resolution numeric camera connected to a computer driving the acquisition software Axiovision (Zeiss, Le Pecq, France).

Techniques: Cell Culture, Inverted Microscopy